BP

GO:0000724

double-strand break repair via homologous recombination

The error-free repair of a double-strand break in DNA in which the broken DNA molecule is repaired using homologous sequences. A strand in the broken DNA searches for a homologous region in an intact chromosome to serve as the template for DNA synthesis. The restoration of two intact DNA molecules results in the exchange, reciprocal or nonreciprocal, of genetic material between the intact DNA molecule and the broken DNA molecule.

ISS

BP

GO:0000737

DNA catabolic process, endonucleolytic

The chemical reactions and pathways resulting in the breakdown of DNA, involving the hydrolysis of internal 3',5'-phosphodiester bonds in one or two strands of deoxyribonucleotides.

IMP

BP

GO:0001934

positive regulation of protein phosphorylation

Any process that activates or increases the frequency, rate or extent of addition of phosphate groups to amino acids within a protein.

IMP

BP

GO:0006974

cellular response to DNA damage stimulus

Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism.

IDA

BP

GO:0008219

cell death

Any biological process that results in permanent cessation of all vital functions of a cell. A cell should be considered dead when any one of the following molecular or morphological criteria is met: (1) the cell has lost the integrity of its plasma membrane; (2) the cell, including its nucleus, has undergone complete fragmentation into discrete bodies (frequently referred to as apoptotic bodies). The cell corpse (or its fragments) may be engulfed by an adjacent cell in vivo, but engulfment of whole cells should not be considered a strict criteria to define cell death as, under some circumstances, live engulfed cells can be released from phagosomes (see PMID:18045538).

IDA|IMP

BP

GO:0016567

protein ubiquitination

The process in which one or more ubiquitin groups are added to a protein.

IDA|IEA

BP

GO:0031297

replication fork processing

The process in which a DNA replication fork that has stalled is to a functional state and replication is restarted. The stalling may be due to DNA damage, DNA secondary structure, bound proteins, dNTP shortage, or other causes.

IDA

BP

GO:0032508

DNA duplex unwinding

The process in which interchain hydrogen bonds between two strands of DNA are broken or 'melted', generating a region of unpaired single strands.

IEA

BP

GO:0035562

negative regulation of chromatin binding

Any process that stops or reduces the frequency, rate or extent of chromatin binding. Chromatin binding is the selective interaction with chromatin, the network of fibers of DNA, protein, and sometimes RNA, that make up the chromosomes of the eukaryotic nucleus during interphase.

IEA

BP

GO:0048478

replication fork protection

Any process that prevents the collapse of stalled replication forks.

ISS

BP

GO:0072429

response to intra-S DNA damage checkpoint signaling

A process that occurs in response to signals generated as a result of intra-S DNA damage checkpoint signaling.

IMP

BP

GO:1902231

positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage

Any process that activates or increases the frequency, rate or extent of intrinsic apoptotic signaling pathway in response to DNA damage.

IMP

BP

GO:2000042

negative regulation of double-strand break repair via homologous recombination

Any process that stops, prevents, or reduces the frequency, rate or extent of double-strand break repair via homologous recombination.

IDA

CC

GO:0000785

chromatin

The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.

IDA

CC

GO:0005634

nucleus

A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

IDA

CC

GO:0019005

SCF ubiquitin ligase complex

A ubiquitin ligase complex in which a cullin from the Cul1 subfamily and a RING domain protein form the catalytic core; substrate specificity is conferred by a Skp1 adaptor and an F-box protein. SCF complexes are involved in targeting proteins for degradation by the proteasome. The best characterized complexes are those from yeast and mammals (with core subunits named Cdc53/Cul1, Rbx1/Hrt1/Roc1).

IDA

MF

GO:0003678

DNA helicase activity

Catalysis of the reaction: NTP + H2O = NDP + phosphate, to drive the unwinding of a DNA helix.

IDA|IMP

MF

GO:0003690

double-stranded DNA binding

Interacting selectively and non-covalently with double-stranded DNA.

IDA

MF

GO:0003697

single-stranded DNA binding

Interacting selectively and non-covalently with single-stranded DNA.

IDA

MF

GO:0004003

ATP-dependent DNA helicase activity

Catalysis of the reaction: ATP + H2O = ADP + phosphate; this reaction drives the unwinding of the DNA helix.

IEA

MF

GO:0005515

protein binding

Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).

IPI

MF

GO:0005524

ATP binding

Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.

IEA

MF

GO:0015616

DNA translocase activity

Catalysis of the reaction: ATP + H2O = ADP + phosphate, to drive movement along a single- or double-stranded DNA molecule.

IDA

MF

GO:0043138

3'-5' DNA helicase activity

Catalysis of the unwinding of the DNA helix in the direction 3' to 5'.

IDA