BP

GO:0001731

formation of translation preinitiation complex

The joining of the small ribosomal subunit, ternary complex, and mRNA.

IEA

BP

GO:0006413

translational initiation

The process preceding formation of the peptide bond between the first two amino acids of a protein. This includes the formation of a complex of the ribosome, mRNA, and an initiation complex that contains the first aminoacyl-tRNA.

IC|IDA|TAS

BP

GO:0006446

regulation of translational initiation

Any process that modulates the frequency, rate or extent of translational initiation.

IDA

BP

GO:0075522

IRES-dependent viral translational initiation

Process by which viral mRNA translation is initiated, where a domain in the 5' untranslated region (UTR) of the viral mRNA called an internal ribosome entry site (IRES) binds the host 43S preinitiation complex, circumventing regular cap-dependent translation initiation.

IDA

BP

GO:0075525

viral translational termination-reinitiation

A process which occurs as part of viral mRNA translation which allows expression of a downstream open reading frame (ORF) in a dicistronic mRNA. In this process, ribosomes translate the upstream ORF but following termination, a proportion of 40S subunits remain tethered to the mRNA and go on to re-initiate translation at the start codon of the downstream ORF.

IDA

CC

GO:0005654

nucleoplasm

That part of the nuclear content other than the chromosomes or the nucleolus.

IDA

CC

GO:0005737

cytoplasm

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

IDA

CC

GO:0005829

cytosol

The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

TAS

CC

GO:0005852

eukaryotic translation initiation factor 3 complex

A complex of several polypeptides that plays at least two important roles in protein synthesis: First, eIF3 binds to the 40S ribosome and facilitates loading of the Met-tRNA/eIF2.GTP ternary complex to form the 43S preinitiation complex. Subsequently, eIF3 apparently assists eIF4 in recruiting mRNAs to the 43S complex. The eIF3 complex contains five conserved core subunits, and may contain several additional proteins; the non-core subunits are thought to mediate association of the complex with specific sets of mRNAs.

IDA

CC

GO:0016282

eukaryotic 43S preinitiation complex

A protein complex composed of the 40S ribosomal subunit plus eIF1A, eIF3, and eIF2-GTP-bound methionyl-initiator methionine tRNA.

IEA

CC

GO:0033290

eukaryotic 48S preinitiation complex

A protein complex composed of the small ribosomal subunit, eIF3, eIF1A, methionyl-initiatior methionine and a capped mRNA. The complex is initially positioned at the 5'-end of the capped mRNA.

IEA

CC

GO:0070062

extracellular exosome

A membrane-bounded vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.

IDA

CC

GO:0071541

eukaryotic translation initiation factor 3 complex, eIF3m

An eukaryotic translation initiation factor 3 complex that contains the PCI-domain protein eIF3m.

IEA

MF

GO:0000166

nucleotide binding

Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose.

IEA

MF

GO:0003743

translation initiation factor activity

Functions in the initiation of ribosome-mediated translation of mRNA into a polypeptide.

IC|IDA

MF

GO:0005515

protein binding

Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).

IPI

MF

GO:0031369

translation initiation factor binding

Interacting selectively and non-covalently with a translation initiation factor, any polypeptide factor involved in the initiation of ribosome-mediated translation.

IEA

MF

GO:0032947

protein complex scaffold

Functions to provide a physical support for the assembly of a multiprotein complex.

TAS