BP

GO:0000380

alternative mRNA splicing, via spliceosome

The process of generating multiple mRNA molecules from a given set of exons by differential use of exons from the primary transcript(s) to form multiple mature mRNAs that vary in their exon composition.

IDA

BP

GO:0000395

mRNA 5'-splice site recognition

Recognition of the intron 5'-splice site by components of the assembling spliceosome.

IDA

BP

GO:0000398

mRNA splicing, via spliceosome

The joining together of exons from one or more primary transcripts of messenger RNA (mRNA) and the excision of intron sequences, via a spliceosomal mechanism, so that mRNA consisting only of the joined exons is produced.

IC|TAS

BP

GO:0001701

in utero embryonic development

The process whose specific outcome is the progression of the embryo in the uterus over time, from formation of the zygote in the oviduct, to birth. An example of this process is found in Mus musculus.

IEA

BP

GO:0006369

termination of RNA polymerase II transcription

The process in which the synthesis of an RNA molecule by RNA polymerase II using a DNA template is completed.

TAS

BP

GO:0006376

mRNA splice site selection

Selection of a splice site by components of the assembling spliceosome.

TAS

BP

GO:0006397

mRNA processing

Any process involved in the conversion of a primary mRNA transcript into one or more mature mRNA(s) prior to translation into polypeptide.

TAS

BP

GO:0006405

RNA export from nucleus

The directed movement of RNA from the nucleus to the cytoplasm.

TAS

BP

GO:0006406

mRNA export from nucleus

The directed movement of mRNA from the nucleus to the cytoplasm.

TAS

BP

GO:0031124

mRNA 3'-end processing

Any process involved in forming the mature 3' end of an mRNA molecule.

TAS

BP

GO:0033120

positive regulation of RNA splicing

Any process that activates or increases the frequency, rate or extent of RNA splicing.

IEA

BP

GO:0048709

oligodendrocyte differentiation

The process in which a relatively unspecialized cell acquires the specialized features of an oligodendrocyte. An oligodendrocyte is a type of glial cell involved in myelinating the axons of neurons in the central nervous system.

IEA

BP

GO:0060048

cardiac muscle contraction

Muscle contraction of cardiac muscle tissue.

IEA

BP

GO:0097421

liver regeneration

The regrowth of lost or destroyed liver.

IEA

CC

GO:0005634

nucleus

A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

IDA

CC

GO:0005654

nucleoplasm

That part of the nuclear content other than the chromosomes or the nucleolus.

IDA|TAS

CC

GO:0005737

cytoplasm

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

IEA

CC

GO:0016607

nuclear speck

A discrete extra-nucleolar subnuclear domain, 20-50 in number, in which splicing factors are seen to be localized by immunofluorescence microscopy.

IDA

CC

GO:0035145

exon-exon junction complex

A multi-subunit complex deposited by the spliceosome upstream of messenger RNA exon-exon junctions. The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay.

IDA

CC

GO:0070062

extracellular exosome

A membrane-bounded vesicle that is released into the extracellular region by fusion of the limiting endosomal membrane of a multivesicular body with the plasma membrane. Extracellular exosomes, also simply called exosomes, have a diameter of about 40-100 nm.

IDA

CC

GO:0071013

catalytic step 2 spliceosome

A spliceosomal complex that contains three snRNPs, including U5, bound to a splicing intermediate in which the first catalytic cleavage of the 5' splice site has occurred. The precise subunit composition differs significantly from that of the catalytic step 1, or activated, spliceosome, and includes many proteins in addition to those found in the associated snRNPs.

IDA

MF

GO:0000166

nucleotide binding

Interacting selectively and non-covalently with a nucleotide, any compound consisting of a nucleoside that is esterified with (ortho)phosphate or an oligophosphate at any hydroxyl group on the ribose or deoxyribose.

IEA

MF

GO:0003723

RNA binding

Interacting selectively and non-covalently with an RNA molecule or a portion thereof.

IDA|IPI

MF

GO:0003729

mRNA binding

Interacting selectively and non-covalently with messenger RNA (mRNA), an intermediate molecule between DNA and protein. mRNA includes UTR and coding sequences, but does not contain introns.

IDA

MF

GO:0005515

protein binding

Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).

IPI

MF

GO:0043422

protein kinase B binding

Interacting selectively and non-covalently with protein kinase B, an intracellular kinase that is important in regulating glucose metabolism.

IEA

MF

GO:0044822

poly(A) RNA binding

Interacting non-covalently with a poly(A) RNA, a RNA molecule which has a tail of adenine bases.

IDA

MF

GO:0050733

RS domain binding

Interacting selectively and non-covalently with an RS domain of a protein; RS domains are usually highly phosphorylated and characterized by the presence of arginine (R)/serine (S) dipeptides. The RS domain promotes protein-protein interactions and directs subcellular localization and, in certain situations, nucleocytoplasmic shuttling of individual SR proteins. They also play a role in splicing.

IEA