BP

GO:0000070

mitotic sister chromatid segregation

The cell cycle process in which replicated homologous chromosomes are organized and then physically separated and apportioned to two sets during the mitotic cell cycle. Each replicated chromosome, composed of two sister chromatids, aligns at the cell equator, paired with its homologous partner. One homolog of each morphologic type goes into each of the resulting chromosome sets.

IMP

BP

GO:0000086

G2/M transition of mitotic cell cycle

The mitotic cell cycle transition by which a cell in G2 commits to M phase. The process begins when the kinase activity of M cyclin/CDK complex reaches a threshold high enough for the cell cycle to proceed. This is accomplished by activating a positive feedback loop that results in the accumulation of unphosphorylated and active M cyclin/CDK complex.

IDA|TAS

BP

GO:0000122

negative regulation of transcription from RNA polymerase II promoter

Any process that stops, prevents, or reduces the frequency, rate or extent of transcription from an RNA polymerase II promoter.

IMP

BP

GO:0000281

mitotic cytokinesis

A cell cycle process that results in the division of the cytoplasm of a cell after mitosis, resulting in the separation of the original cell into two daughter cells.

IDA

BP

GO:0000910

cytokinesis

The division of the cytoplasm and the plasma membrane of a cell and its partitioning into two daughter cells.

IDA|IMP

BP

GO:0001578

microtubule bundle formation

A process that results in a parallel arrangement of microtubules.

IDA

BP

GO:0006468

protein phosphorylation

The process of introducing a phosphate group on to a protein.

IDA

BP

GO:0007062

sister chromatid cohesion

The cell cycle process in which the sister chromatids of a replicated chromosome become tethered to each other.

TAS

BP

GO:0007067

mitotic nuclear division

A cell cycle process comprising the steps by which the nucleus of a eukaryotic cell divides; the process involves condensation of chromosomal DNA into a highly compacted form. Canonically, mitosis produces two daughter nuclei whose chromosome complement is identical to that of the mother cell.

IDA|IMP

BP

GO:0007077

mitotic nuclear envelope disassembly

The cell cycle process in which the controlled breakdown of the nuclear envelope during mitotic cell division occurs.

TAS

BP

GO:0007094

mitotic spindle assembly checkpoint

A cell cycle checkpoint that delays the metaphase/anaphase transition of a mitotic nuclear division until the spindle is correctly assembled and chromosomes are attached to the spindle.

IMP

BP

GO:0007346

regulation of mitotic cell cycle

Any process that modulates the rate or extent of progress through the mitotic cell cycle.

IMP

BP

GO:0008283

cell proliferation

The multiplication or reproduction of cells, resulting in the expansion of a cell population.

TAS

BP

GO:0010800

positive regulation of peptidyl-threonine phosphorylation

Any process that increases the frequency, rate or extent of peptidyl-threonine phosphorylation. Peptidyl-threonine phosphorylation is the phosphorylation of peptidyl-threonine to form peptidyl-O-phospho-L-threonine.

IMP

BP

GO:0016321

female meiosis chromosome segregation

The cell cycle process in which genetic material, in the form of chromosomes, is organized and then physically separated and apportioned to two or more sets during the meiotic cell cycle in a female.

IEA

BP

GO:0016567

protein ubiquitination

The process in which one or more ubiquitin groups are added to a protein.

IDA

BP

GO:0018105

peptidyl-serine phosphorylation

The phosphorylation of peptidyl-serine to form peptidyl-O-phospho-L-serine.

IDA

BP

GO:0030071

regulation of mitotic metaphase/anaphase transition

Any process that modulates the frequency, rate or extent of the cell cycle process in which a cell progresses from metaphase to anaphase during mitosis, triggered by the activation of the anaphase promoting complex by Cdc20/Sleepy homolog which results in the degradation of Securin.

IMP

BP

GO:0031145

anaphase-promoting complex-dependent catabolic process

The chemical reactions and pathways resulting in the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of ubiquitin, with ubiquitin-protein ligation catalyzed by the anaphase-promoting complex, and mediated by the proteasome.

TAS

BP

GO:0031572

G2 DNA damage checkpoint

A cell cycle checkpoint that detects and negatively regulates progression from G2 to M phase in the cell cycle in response to DNA damage.

IDA

BP

GO:0031648

protein destabilization

Any process that decreases the stability of a protein, making it more vulnerable to degradative processes or aggregation.

IDA

BP

GO:0032436

positive regulation of proteasomal ubiquitin-dependent protein catabolic process

Any process that activates or increases the frequency, rate or extent of the breakdown of a protein or peptide by hydrolysis of its peptide bonds, initiated by the covalent attachment of ubiquitin, and mediated by the proteasome.

IMP

BP

GO:0042787

protein ubiquitination involved in ubiquitin-dependent protein catabolic process

The process in which a ubiquitin group, or multiple groups, are covalently attached to the target protein, thereby initiating the degradation of that protein.

TAS

BP

GO:0043066

negative regulation of apoptotic process

Any process that stops, prevents, or reduces the frequency, rate or extent of cell death by apoptotic process.

IMP

BP

GO:0043393

regulation of protein binding

Any process that modulates the frequency, rate or extent of protein binding.

IMP

BP

GO:0045143

homologous chromosome segregation

The cell cycle process in which replicated homologous chromosomes are organized and then physically separated and apportioned to two sets during the first division of the meiotic cell cycle. Each replicated chromosome, composed of two sister chromatids, aligns at the cell equator, paired with its homologous partner; this pairing off, referred to as synapsis, permits genetic recombination. One homolog (both sister chromatids) of each morphologic type goes into each of the resulting chromosome sets.

IEA

BP

GO:0045184

establishment of protein localization

The directed movement of a protein to a specific location.

IMP

BP

GO:0045736

negative regulation of cyclin-dependent protein serine/threonine kinase activity

Any process that stops, prevents, or reduces the frequency, rate or extent of cyclin-dependent protein serine/threonine kinase activity.

IMP

BP

GO:0045862

positive regulation of proteolysis

Any process that activates or increases the frequency, rate or extent of the hydrolysis of a peptide bond or bonds within a protein.

IDA

BP

GO:0051297

centrosome organization

A process that is carried out at the cellular level which results in the assembly, arrangement of constituent parts, or disassembly of a centrosome, a structure comprised of a pair of centrioles and peri-centriolar material from which a microtubule spindle apparatus is organized.

IMP

BP

GO:0051437

positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition

Any process that activates, maintains or increases the rate of ubiquitin ligase activity that contributes to the regulation of the mitotic cell cycle phase transition.

TAS

BP

GO:0051439

regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle

A cell cycle process that modulates the frequency, rate or extent of ubiquitin ligase activity that contributes to the mitotic cell cycle.

TAS

BP

GO:0051443

positive regulation of ubiquitin-protein transferase activity

Any process that activates, maintains or increases the rate of ubiquitin transferase activity.

IMP

BP

GO:0051726

regulation of cell cycle

Any process that modulates the rate or extent of progression through the cell cycle.

TAS

BP

GO:0070194

synaptonemal complex disassembly

The controlled breakdown of a synaptonemal complex.

IEA

BP

GO:0071168

protein localization to chromatin

Any process in which a protein is transported to, or maintained at, a part of a chromosome that is organized into chromatin.

IDA

BP

GO:1900182

positive regulation of protein localization to nucleus

Any process that activates or increases the frequency, rate or extent of protein localization to nucleus.

TAS

BP

GO:1901673

regulation of mitotic spindle assembly

Any process that modulates the frequency, rate or extent of mitotic spindle assembly.

IDA

BP

GO:1902749

regulation of cell cycle G2/M phase transition

Any process that modulates the frequency, rate or extent of cell cycle G2/M phase transition.

TAS

BP

GO:1904668

positive regulation of ubiquitin protein ligase activity

Any process that activates or increases the frequency, rate or extent of ubiquitin protein ligase activity.

IDA

CC

GO:0000776

kinetochore

A multisubunit complex that is located at the centromeric region of DNA and provides an attachment point for the spindle microtubules.

IDA

CC

GO:0000785

chromatin

The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome.

IEA

CC

GO:0000795

synaptonemal complex

A proteinaceous scaffold found between homologous chromosomes during meiosis.

IEA

CC

GO:0000922

spindle pole

Either of the ends of a spindle, where spindle microtubules are organized; usually contains a microtubule organizing center and accessory molecules, spindle microtubules and astral microtubules.

IDA

CC

GO:0000942

condensed nuclear chromosome outer kinetochore

The region of a condensed nuclear chromosome kinetochore most external to centromeric DNA; this outer region mediates kinetochore-microtubule interactions.

IDA

CC

GO:0005634

nucleus

A membrane-bounded organelle of eukaryotic cells in which chromosomes are housed and replicated. In most cells, the nucleus contains all of the cell's chromosomes except the organellar chromosomes, and is the site of RNA synthesis and processing. In some species, or in specialized cell types, RNA metabolism or DNA replication may be absent.

IDA

CC

GO:0005654

nucleoplasm

That part of the nuclear content other than the chromosomes or the nucleolus.

TAS

CC

GO:0005730

nucleolus

A small, dense body one or more of which are present in the nucleus of eukaryotic cells. It is rich in RNA and protein, is not bounded by a limiting membrane, and is not seen during mitosis. Its prime function is the transcription of the nucleolar DNA into 45S ribosomal-precursor RNA, the processing of this RNA into 5.8S, 18S, and 28S components of ribosomal RNA, and the association of these components with 5S RNA and proteins synthesized outside the nucleolus. This association results in the formation of ribonucleoprotein precursors; these pass into the cytoplasm and mature into the 40S and 60S subunits of the ribosome.

IDA

CC

GO:0005737

cytoplasm

All of the contents of a cell excluding the plasma membrane and nucleus, but including other subcellular structures.

IDA

CC

GO:0005813

centrosome

A structure comprised of a core structure (in most organisms, a pair of centrioles) and peripheral material from which a microtubule-based structure, such as a spindle apparatus, is organized. Centrosomes occur close to the nucleus during interphase in many eukaryotic cells, though in animal cells it changes continually during the cell-division cycle.

IDA

CC

GO:0005819

spindle

The array of microtubules and associated molecules that forms between opposite poles of a eukaryotic cell during mitosis or meiosis and serves to move the duplicated chromosomes apart.

IDA

CC

GO:0005829

cytosol

The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.

TAS

CC

GO:0005876

spindle microtubule

Any microtubule that is part of a mitotic or meiotic spindle; anchored at one spindle pole.

IDA

CC

GO:0015630

microtubule cytoskeleton

The part of the cytoskeleton (the internal framework of a cell) composed of microtubules and associated proteins.

IDA

CC

GO:0030496

midbody

A thin cytoplasmic bridge formed between daughter cells at the end of cytokinesis. The midbody forms where the contractile ring constricts, and may persist for some time before finally breaking to complete cytokinesis.

IDA

CC

GO:0051233

spindle midzone

The area in the center of the spindle where the spindle microtubules from opposite poles overlap.

IDA

MF

GO:0004672

protein kinase activity

Catalysis of the phosphorylation of an amino acid residue in a protein, usually according to the reaction: a protein + ATP = a phosphoprotein + ADP.

IDA

MF

GO:0004674

protein serine/threonine kinase activity

Catalysis of the reactions: ATP + protein serine = ADP + protein serine phosphate, and ATP + protein threonine = ADP + protein threonine phosphate.

EXP|IDA|IMP|TAS

MF

GO:0005515

protein binding

Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).

IPI

MF

GO:0005524

ATP binding

Interacting selectively and non-covalently with ATP, adenosine 5'-triphosphate, a universally important coenzyme and enzyme regulator.

IEA

MF

GO:0008017

microtubule binding

Interacting selectively and non-covalently with microtubules, filaments composed of tubulin monomers.

IDA

MF

GO:0010997

anaphase-promoting complex binding

Interacting selectively and non-covalently with an anaphase-promoting complex. A ubiquitin ligase complex that degrades mitotic cyclins and anaphase inhibitory protein, thereby triggering sister chromatid separation and exit from mitosis.

IPI

MF

GO:0016301

kinase activity

Catalysis of the transfer of a phosphate group, usually from ATP, to a substrate molecule.

TAS

MF

GO:0019901

protein kinase binding

Interacting selectively and non-covalently with a protein kinase, any enzyme that catalyzes the transfer of a phosphate group, usually from ATP, to a protein substrate.

IPI